Part:BBa_K211002:Design

RI7-odr10 chimeric GPCR

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 761
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Designed with PCR. Primer Sequence

P1 RI7-N F ATATCTGAATTCGCCACCATGGAACGAAGGAACCAC

P2 RI7-C R GTATACGCGGCCGCACCAATTTTGCTGCCTTTG

P3 RI7-N R GAGAAGAA ATACATGGGTTTGTGGAG

P4 Odr-10 F CCATGTAT TTCTTCTCAGTATTCTCG

P5 Odr-10 R GCAGTA AATGATCAGAATCAGAATGAG

P6 RI7-C F GATCATT TACTGCTTGCGCAACC

BioBrick F ATATCT TCTAGATGGAACGAAGGAACCAC

BioBrick R GTATACACTAGTA TTA TTA ACCAATTTTGCTGCC

We would like to thank Professor Piali Sengupta, Department of Biology, Brandeis University for the kind gift of the odr-10 cDNA, and Professor Stuart Firestein, Department of Biological Sciences, Columbia University for the kind gift of the rat I7 olfactory receptor cDNA.

Source

Caenorhabditis elegans olfactory receptor Odr-10 (GenBank: U49449.1)

Rat olfactory protein, clone I7 (GenBank: M64386.1)

References

Radhika, V. et al . 2007. Chemical sensing of DNT by engineered olfactory yeast strain. Nat. Chem. Biol. 3 : 325–330